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KMID : 0812020060120020151
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Korean Journal of Neurogastroenterology and Motility
2006 Volume.12 No. 2 p.151 ~ p.156
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Modulation of Pacemanker Ca Activity by Serotonin in the Cultured Interstitial Cells of the Cajal Clusters Isolated from Mouse Small Intestine
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Shin Dong-Hyun
Rhee Poong-Lyul
Kim Jae-Jun
Sohn Hee Jung
Lee Ji-Youn
So In-Suk
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Abstract
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Background/Aims: Interstitial cells of Cajal (ICCs) are considered to be pacemaker cells in the gastrointestinal tracts and Ca2+ oscillation in ICCs is intimately linked to the pacemaker function. Although serotonin is one of most importance substance involved in gastrointestinal motility, it’s effects on the ICCs are not known. We wanted to study the effect of serotonin on Ca2+ oscillation in ICCs.
Methods: Using cultured cell clusters isolated from the mouse ileum, we measured spontaneous Ca2+ oscillation as an index of pacemaker activity in the ICCs. The cultured cell clusters showed spontaneous contraction and they contained c-Kit immunopositive cells. We visualized the changes in intracellular Ca2 concentration in the ICCs within the cultured cell clusters that were loaded with a fluorescent Ca2 indicator, fura-2.
Results: There was no significant change in the fluorescence emission intensity and spike frequency between the baseline and after the addition of TTX. The addition of serotonin in the buffer solution caused an increase in the fluorescence emission intensity and the spike frequency per minute of Ca2+ oscillations. The addition of serotonin in the presence of TTX caused increases in the spike frequency per minute of the calcium oscillation.
Conclusions: These results suggest that serotonin may modulate the pacemaker Ca2+ activity in ICCs by the TTX-resistant, direct serotonergic pathway. (Kor J Neurogastroenterol Motil 2006;12:151-156)
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KEYWORD
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Interstitial cells of Cajal, Serotonin, Modulation
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